Quantitative phase imaging (QPI) becomes more and more popular in biomedical imaging, especially in optical microscopy. Unlike other methods relying on fluorescence of contrast agents, incorporated into the sample, QPI extracts phase and amplitude directly from the optical field transmitted or reflected by the object, rendering sample labeling optional […]
We have been developing, for about 15 years now, a generalization of QPI called Tomographic Diffractive Microscopy (TDM). By varying the object’s illumination conditions, it is possible to obtain a 3D reconstruction of its complex refractive index, with improved resolution compared to conventionnal QPI approaches […] These methods offer an interesting alternative to flurorescence microscopy, but suffer from a lack of chemical selectivity in the reconstructed information. Indeed, very different structures may have a similar refractive index. The aim of this innovative PhD proposal is to develop new approaches, in order to restore selectivity to tomographic images […]
Another possible approach is based on multispectral or hyperspectral imaging, which is
the topic of this PhD proposal. In particular, it has already been proven that the variation of absorption with wavelength enables chemical species to be distinguished at the micrometric scale […]
